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OPERATION PROCEDURES FOR PHILIPS/FEI CM-20
TRANSMISSION ELECTRON MICROSCOPE
General Comments
Normally the microscope will be left in the power ON (standby ready to operate)
condition; i.e., all circuits will be continuously energized and the microscope will be under full
vacuum (MAIN, VACUUM, HIGH TENSION lamps on the right control panel are all lit if the
PANEL DIM is pressed). If it is not in this condition do not operate the microscope. Seek help
from authorized personnel.
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(2) Hold the specimen holder with the airlock-opening pin up. Carefully insert the specimen holder into the airlock entry and slide it in until stop. At this point, pre-pumping of the airlock commences as indicated by illumination of the red airlock indicator light. (3) After hearing a click sound, grape the specimen holder and turn clockwise, the specimen holder will slide into a groove in the airlock by vacuum. (4) Wait until the airlock indicator light extinguishes, then rotate the hol
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(4) Make sure the camera cover is correctly placed, and then press CAM AIR again to pump the camera chamber. You should be able to hear the mechanical pump starting. Pull on the camera lid to ensure the vacuum is pulling. (5) Press READY, then TEM CAMERA, and CAM INIT. (6) Press RESET to reset film number (to 36), and press READY. (7) Press MODES and then READY to go to start-up page. (8) Develop your films (negatives). Be careful not to contaminate any chemicals onto the unexposed f
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ROUTINE ALIGNMENT PROCEDURES FOR PHILIPS CM-20 TEM The following protocol is not intended to be specific. It is intended to be used as a mean to refresh one’s memory in carry out step by step procedures which are necessary to obtain reasonably good results with the transmission electron microscopes. More specific information can be obtained from the instruction manual from the Microscopist in Charge or the Center Director. Please do not hesitate to ask about anything you do not unders
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(6) Set voltage at 20. Push HIGH TENSION button (the green LED should light) Slowly increase voltage from 20 kV to 160 kV using soft keys (wait 1 minute between each step). (7) After voltage reaches 160 kV, press FREE CONTROL soft key. Use focus knob, slowly increase voltage from 160kV to 200kV. If it arcs (loud popping sound), use focus step size knob to decrease voltage increment. You may also increase voltage to 200 kV by directly press 200 if the microscope has been conditione
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by first making crossover image with INTENSITY knob, then open up condenser II (INTENSITY)/expand the beam and close condenser II on the opposite side of crossover. If the beam appears to swing this indicates the aperture is not properly centered. Center the aperture by moving the X and Y controls on the aperture housing. (5) Repeat the last step until the illuminated area remains centered and becomes concentrically smaller or larger on changing the setting of the INTENSITY knob.
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(4) Condenser lens astigmatism correction - Press STIG to activate stigmator control. - Press COND soft key so that it is highlighted (the default is OBJ). - Condense the beam. - Use MULTIFUNCTION knobs to correct the astigmatism. The beam should be round when changing the setting of the INTENSITY knob, or the filament image will be sharpest if filament is under-saturated. - To see the sharp image of filament, three conditions, under-saturated, crossover,
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- Press ALGN to de-activate. (3) Voltage centering - Place a more or less symmetrical image to the center. - Condense the beam at the center of screen, and spread the beam. - Press ROT CENTER and VOLT soft keys in ALGN. - Make sure it is in the VOLT mode (check bottom right soft key). - Determine the voltage center from the movement of the entire image and match the center of image movement with that of the fluorescent screen by adjusting MULTIFUNCTION knobs. - If necessary, increase fo
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(5) Change camera length (by changing MAGNIFICATION knob) to 44.0 mm. (6) Realign the diffraction pattern. (7) Repeat steps 4 to 6 until the transmitted spot remains at (near) the center while changing camera length between 44.0 mm to 3.2 m. (8) Deactivate ALGN, and depress D to get out of diffraction mode. (iv) Insertion and centering objective aperture (1) Move to the edge of the sample. (2) Make sure aperture is out. (3) Press D to get to diffraction mode. (4) Put objec
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(2) Set a magnification at least 3 or 4 times higher than that will used for photographing. (3) Obtain the thinnest over-focus (Fresnel) fringe that can be clearly seen in the binocular, with beam spread. Caution: make sure you focus the binocular on the viewing screen. (4) Adjust the OJB STIG X and Y (MULTIFUNCTION keys) until the fringe follows the outline of the hole uniformly and symmetrically. (5) Use finest focus step, go through focus series to check astigmatism correction.
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WAC 09/28/02 VI. COMPUTER ALIGNMENT AND ASTGMATISM CORRECTION FOR 200 kV 1. Preses PARAMETERS on TEM bright field page 3 times 2. Press REMOTE CONTROL to highlight it (This will allow the computer to talk to the TEM). 3. Turn on the PC monitor (on/off switch on back of monitor). 4. If computer is already in execution mode, go to step 6 5. At the prompt, C:\RCM > type CMONITOR 6. Press F4 to set alignment. When program asks for a file name, choose the file tha
