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Confocal Laser Scanning
Biological Microscope
FV1000
FLUOVIEW
FLUOVIEW—Always Evolving
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FLUOVIEW–—From Olympus is Open FLUOVIEW—More Advanced than Ever The Olympus FLUOVIEW FV1000 confocal laser scanning microscope delivers efficient and reliable performance together with the high resolution required for multi-dimensional observation of cell and tissue morphology, and precise molecular localization. The FV1000 incorporates the industry’s first dedicated laser light stimulation scanner to achieve simultaneous targeted laser stimulation and imaging for real-time visualization of rapi
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Imaging to Analysis ing up New Worlds From Imaging to Analysis FV1000 Advanced Deeper Imaging with High Resolution FV1000MPE 2
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Advanced FLUOVIEW Systems Enhance the Power of Your Research Superb Optical Systems Set the Standard for Accuracy and Sensitivity. Two types of detectors deliver enhanced accuracy and sensitivity, and are paired with a new objective with low chromatic aberration, to deliver even better precision for colocalization analysis. These optical advances boost the overall system capabilities and raise performance to a new level. Imaging, Stimulation and Measurement— Advanced Analytical Methods for Qu
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Excellent Precision, Sensitivity and Stability. FLUOVIEW Enables Precise, Bright Imaging with Minimum Phototox Main scanner Barrier filter Grating Grating Laser combiner LD635 Broadband fiber * Confocal pinhole LD559 AOTF LD473 AOTF LD405 Broadband fiber Laser combiner/Fiber Scanners/Detection Laser Combiner Diode Laser High Sensitivity Detection System Greater stability, longer service life and High-sensitivity and high S/N ratio optical lower operating cost are achieved using performance is a
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Technology / Hardware icity. SIM Scanner * * Option Microscope PMT PMT PMT Specimen Galvanometer scanning mirrors UIS2 objectives Galvanometer Pupil scanning mirrors projection lens Optical System Samples and Specimens Motorized Microscopes UIS2 Objectives Supports a Wide Range of Samples and Specimens Compatible with Olympus IX81 inverted Olympus UIS2 objectives offer world- microscope, BX61WI focusing nosepiece leading, infinity-corrected optics that Tissue culture dishes, slide chambers,
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Two Versions of Light Detection System that Set New Standards for Optical Performance. Spectral Based Detection Flexibility and High Sensitivity Spectral detection using gratings for 2 nm wavelength resolution and image acquisition matched to fluorescence wavelength peaks. User adjustable bandwidth of emission spectrum for acquiring bright images with minimal cross- talk. EGFP–EYFP Fluorescence Separation Precise Spectral Imaging 2,600 2,400 The spectral detection unit uses a grating method th
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Technology / Hardware SIM Scanner Unit for Simultaneous Laser Light Stimulation and Imaging. SIM (Simultaneous) Scanner Unit Combines the main scanner with a dedicated laser light Lasers are used for both imaging stimulation scanner for investigating the trafficking of fluorescent- and laser light stimulation. labeled molecules and marking of specific live cells. Simultaneous Laser Light Stimulation and Imaging Performs simultaneous laser light stimulation and imaging to acquire images of immedi
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New Objective with Low Chromatic Aberration Delivers World-Leading Imaging Performance. NEW NEW Low Chromatic Aberration Objective Best Reliability for Colocalization Analysis A new high NA oil-immersion objective minimizes chromatic aberration in the 405–650 nm region for enhanced imaging performance and image resolution at 405 nm. Delivers a high degree of correction for both lateral and axial chromatic aberration, for acquisition of 2D and 3D images with excellent and reliable accuracy, and i
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Technology / Hardware Exceptional Resolution for Imaging of Cytoplasmic Membrane and Areas Deep Within Living Specimen. TIRFM (Total Internal Reflection Fluorescence Microscope) System Switchable between Confocal and TIRFM Imaging Switchable between confocal and TIRFM imaging for localization of proteins on the cytoplasmic membrane surface and acquisition of sectioning images within cells. Software Control of TIRF Illumination Built-in laser provides TIRF illumination. Software can be used to t
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User-Friendly Software to Support Your Research. Configurable Emission Wide Choice of Scanning Modes Image Acquisition by Application Wavelength Several available scanning modes User-friendly icons offer quick access to including ROI, point and high-speed functions, for image acquisition according Select the dye name to set the optimal bidirectional scanning. to the application (XYZ, XYT, XYZT, XYλ, filters and laser lines. XYλT). Time Controller Precisely synchronizes different experimental pro
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Technology / Hardware Optional Software with Broad Functionality. Diffusion Measurement Package For analysis of intracellular molecular interactions, signal transduction and other processes, by determining standard diffusion coefficients. Supports a wide range of diffusion analysis using point FCS, RICS and FRAP. Multi Stimulation Software Configure multiple stimulation points and conditions for laser light stimulation synchronized with imaging, for detailed analysis of the connectivity of cell
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Broad Application Support and Sophisticated Experiment Control. Multi-Color Imaging Measurement 3D/4D Light Stimulation Volume Rendering Multi- Dimensional Colocalization Time-Lapse FRET 3D Mosaic Imaging 13
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Application Measurement Diffusion measurement and molecular interaction analysis. Light Stimulation FRAP/FLIP/Photoactivation/Photoconversion/Uncaging. Multi-Dimensional Time-Lapse Long-term and multiple point. 3D Mosaic Imaging High resolution images stitched to cover a large area. Multi-Color Imaging Full range of laser wavelengths for imaging of diverse fluorescent dyes and proteins. 3D/4D Volume Rendering One-click 3D/4D image construction from acquired XYZ/T images. Change the angle of 3D
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Diffusion Measurement Package 1.5 This optional software module enables data acquisition and analysis to investigate the molecular interaction and concentrations by calculating the diffusion coefficients of molecules within the cell. 1 130 Diverse analysis methods (RICS/ccRICS, point FCS/point FCCS and FRAP) cover a wide range of molecular 0.5 sizes and speeds. 125 Pixels 0 105 125 105 130 Pixels RICS—Raster Imaging Correlation Spectroscopy Raster image correlation spectroscopy (RICS) is a new
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Application/ Molecular Interaction Analysis RICS Application and Principles Comparison of Diffusion Coefficients for EGFP Fusion Proteins Near to Cell Membranes and In Cytoplasm RICS can be used to designate and analyze regions of interest At cytoplasmic membrane In cytoplasm 2 2 based on acquired images. Diffusion coefficient D =0.98 µm /s Diffusion coefficient D =3.37 µm /s EGFP is fused at protein kinase C (PKC) for visualization, using live cells to analyze the translocation with RICS. The d
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Laser Light Stimulation The SIM scanner system combines the main scanner with a laser light stimulation scanner. Control of the two independent beams enables simultaneous stimulation and imaging, to capture reactions during stimulation. Multi-stimulation software is used to continuously stimulate multiple points with laser light for simultaneous imaging of the effects of stimulation on the cell. FLIP—Fluorescence Loss in Photobleaching Fluorescence loss in photobleaching (FLIP) combines imaging
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Application/ Molecular Interaction Analysis Photoconversion The Kaede protein is a typical photoconvertible protein, which is a specialized fluorescent protein that changes color when exposed to light of a specific wavelength. When the Kaede protein is exposed to laser light, its fluorescence changes from green to red. This phenomenon can be used to mark individual Kaede-expressing target cells among a group of cells, by exposing them to laser light. 450 nm laser light 405 nm 405 nm Before Stimu
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P2 Multi-Dimensional Time-Lapse P1 P3 The FV1000 can be used for ideal multi-dimensional time-lapse imaging during confocal observation, using multi-area time-lapse software to control the motorized XY stage and focus compensation. P4 P5 Significantly Improved Long Time-Lapse Throughput Equipped with motorized XY stage for repeated image acquisition from multiple points scattered across a wide area. The system efficiently analyzes changes over time of cells in several different areas capturing,
