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2/27/07 PhotoScan 2001 instructions
Z/I Imaging PhotoScan 2001 scanner instructions (5 pages total)
Last Updated February 27, 2007
Original Document By Amy McGough
Updates by Paul Chipman
All first-time users should arrange for training from a qualified user from their working group to
receive training prior to scanning. AFTER users have been trained and are competent using the
scanner, they will be given access to Lilly B-216.
Bulbs that come with scanner are different than ol
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2/27/07 PhotoScan 2001 instructions Before scanning: 1) Confirm that the scanner is not currently in use. The scanner should be turned off after every scanning session, unless the next person scanning has showed up before the last person finishes. 2) Enter the date, your name, your supervisor’s name into the log. (Complete log entry after scanning – including number of micrographs scanned and scanner performance.) How to turn on the scanner: 1) Turn scanner on first (b
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2/27/07 PhotoScan 2001 instructions 2) Load electron micrograph(s) emulsion side down between glass plates. Please note: the glass plates should never touch each other – there should always be a negative or the Zeiss plastic insert between the plates. WEAR GLOVES! Be extremely careful lifting the upper glass plate. Rest the plate on the two spongy pads that are on the underside of the raised scanner lid. The spongy pads do not grip the glass plate very tightly, therefore mak
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2/27/07 PhotoScan 2001 instructions How to load configurations: 1) Under “File” menu, select “New Scan” “Scan Properties” window appears you should see three tabs: scan setup, film/output, camera definition 2) Under scan setup, select a parameters file from available list: for example, “cryoEM.par” Check settings for Scan Mode, should be “Gray Scale” “8 bit” Adjust resolution (7, 14, etc etc to 224 microns; use 224 for low resolution scan) Tile size (256) Format (tiff uncompresse
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2/27/07 PhotoScan 2001 instructions 4) Under scan Properties window, go to “histogram tab”. Select a generous portion of the histogram (using the mouse, pick the peak and go well past it on either side). The image is adjusted to account for the new range. Hit the “apply” the histogram button. Note: if you cut the histogram too tightly, you will not be able to adjust the contrast/range of the micrograph properly later on. The “reset” button can be used to undo the change at this step
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2/27/07 PhotoScan 2001 instructions Scanner Users, A sign-up system will be implemented for use on the new scanner. Individuals wishing to reserve the scanner can do so by filling in their name and PI in the weekly calendar which is located beside the scanner, in the black binder. Two-hour time slots have been created for use during peak use periods (8am-6pm, Monday-Friday). Each user may reserve two peak period time slots at a time. Once one of the slots has passed, the pers
