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User’s Guide
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TCY 20, 25, 30 & 48
Thermal Cyclers
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Contents 1. Introduction .............................................................................................................4 2. Safety and installation............................................................................................4 3. Cycler TCY specification........................................................................................7 4. User operation and consumables.........................................................................8 Heated lid .
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1. Introduction The Cycler TCY provides the researcher with the means of accurately controlling the temperature profile of samples. It has many scientific applications, including DNA amplification and sequencing. The Cycler TCY can cycle samples between 4°C and 99°C. The Cycler TCY is programmed by means of an integral keypad and LCD display. A program, which can be recalled from memory, consists of: a series of specified temperatures in °C the times for which each specified tempera
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Unpacking When unpacking please ensure that the following have been removed from the packaging: Thermal cycler Power cable The user is advised to keep the original packaging in case the instrument ever needs to be returned for service or repair. Omega Engineering, Inc. accepts no responsibility for damage incurred unless the unit is correctly packed and transported in its original packaging. Please note that the screen of the thermal cycler is easily damaged by sharp objects such as
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Working Conditions The thermal cycler is designed operate under the following conditions: Indoor use o o Ambient temperature range +5 C to +40 C Altitude to 2000m Relative humidity not exceeding 95% Power supply fluctuations not exceeding 10% Over voltage category II IEC 60364-4-443 Pollution degree 2 o Note: The control specifications are quoted at an ambient temperature of 20 C. The specification o o may deteriorate outside an ambient temperature of 10 C to 30 C. Th
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3. Cycler TCY Specification Temperature Temperature range 4°C to 99°C Temperature set point precision 0.1°C Block uniformity (over full range) ± 0.5°C Temperature accuracy ± 0.5°C End of program cooling below ambient Yes Heating/Cooling Rate Maximum heating rate 4.5ºC/s Minimum heating rate 0.1ºC/s Maximum cooling rate 3.0ºC/s Minimum cooling rate 0.1ºC/s Heated Lid Heated lid enable/disable Yes Selectable heated lid temperature 100°C to 115°
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Dimensions Height 185mm Width 185mm Length 330mm Power Power consumption 230W Units marked 100V will work at any voltage between 90 and 110V. The performance will vary and will not necessarily meet the above typical specification at the extremes of voltage. 4. User operation and consumables Heated lid To release the heated lid, lift the lid catch and the lid will open. To close the lid, push down gently until the catch clicks in place. Do not close the lid without tub
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The amount of volume loss you observe and the change in reactant concentrations you can tolerate determine the minimum volume that can be used. Typical volume losses of 1µl in 30 cycles allow the use of samples of 20µl or less. During the final cool-down, a ring of condensation may form above the liquid level but below the top of the sample block. This is normal and does not effect the reaction. 0.5ml Tubes The heated lid mechanism of the Cycler TCY with the 0.5ml block is optimised for fl
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Switching on When you switch on the unit a screen TC 3000 TCY similar to the one shown here will appear briefly. This indicates the software version Omega Engineering and block type installed in the unit. Version X.XX Version X.XX Block Type 48x0.2ml Block 25x0.2ml or 30x0.5ml When the initial screen has disappeared the screen shown here will automatically appear. There are three options: 1. ‘Programs’ for creating and running Methods
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5. Front panel controls The LCD Display The CYCLER TCY has a four line LCD Programs [0] display. The top three lines are Methods [0] Information Information information or program lines, while the bottom line is a prompt line. The active Configuration Configuration line flashes on screen and is shown in [ , to select ] bold in this manual. [ , to select ] The bottom line may cha
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The keys The End, Finish or Exit key The “End” or “Finish” key is used to finish editing programs or to exit a program. It is also used to exit a sequence of key operations. The Up arrow key The “Up arrow” key is used for scrolling up through the lines on the screen. It is also used to increase certain fields when editing. The Down arrow key The “Down arrow” key is used for scrolling down through the lines on the screen. It is also used to decrease certain
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The Down arrow key The “Down arrow” key is used for scrolling down through the lines on the screen. It is also used to decrease certain fields when editing. The Decimal Point and Insert key The “Decimal Point” key is used when editing numeric fields. It is also used as an Insert key during program editing to insert additional steps and other field sets. The Delete key The “Delete” key is used during program editing to delete steps and stages. The Pause
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In the prompt line is equivalent to the “End” key. On a program line means the program is locked; it can be copied and then edited but not edited directly. Up arrow Down arrow Enter Pause The following terms are used when programming the CYCLER TCY: A ‘Stage’ is the name given to the main repeated part of a program Stage containing the ‘Steps’ to be repeated. A program can contain a maximum of 25 stages although typically only one or two are
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Programming Options There are two options available for creating programs on the CYCLER: 1. Copy and edit one of the pre-written templates. 2. Create a new program from the defaults. Option 1: Creating programs using templates There are two pre-written basic PCR templates: a two-step and a three-step template. These can be copied and modified as required. "In denat" stands for Initial Denaturing. 2 STEP TEM 2 STEP PLATE 3 STEP TEMP 3 STEP LATE Heated Lid Heated Lid 105
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A new name can now be given to the copied program (see below). To give a program a name To type in a name for the program, press "Enter" and a cursor will appear in the position shown. Using the keypad, type in the name you wish to give the program. The letters will scroll round with successive presses; for example the “2” key will give ‘A’ with one press; ‘B’ with two presses; ‘C’ with three presses and ‘2’ with four presses. Five presses will ag
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To edit a field or step in an existing program When the unit is switched on the display Methods Programs [0] [0] will show this screen. Information Information If known, type in the ‘Speed dial’ number Configuration Configuration for the required program and press [ , to select ] [ , to select ] “Enter” This will take you directly to the required program. If you do not know the ‘Speed dial’ number, press “Enter” to acc
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To edit a temperature, type in the required temperature. In the example here, the heated lid temperature is being edited. Press “Enter” to accept. Use the “Down Arrow” and/or the “Up Arrow” keys to select the next field or step you wish to edit. To edit a field with on/off settings, first select the field and press “Enter” Use the “Up Arrow” and “Down Arrow” keys to toggle between the settings. Press “Enter” to accept. To edit
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To copy a selected program If you select ‘Copy program’ a screen similar to this will appear and the software will automatically give the program the next number until it is named. Press “Enter” and type in a new name. You will then be able to edit the program as previously described. To delete a selected program If you select ‘Delete program’ a screen similar to this will appear. Press “Enter” to delete the program or “End” to return to the program scre
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Example programs A couple of example thermal cycling programs are shown below. 3-Step PCR with multiple ‘Stages’ Typical 3-Step PCR Heated Lid 105 Heated Lid 105 Preheat Lid on Preheat Lid on Pause off Pause off - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - In denat 94°c 5min In denat 94°c 5min Hot Start off Hot Start off - - - - - - Stage 1 - - - - - - - - - - - - Stage 1 - - - - - - Number Cycles 30 Number Cycles 10 Step 94°c
