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5. Unpacking the Microscope 5. Unpacking the Microscope First, carefully remove all components from the Installation Location transportation and packaging materials. Work with the microscope should be performed in a dust-free room, which is free of vapors (oil, chemicals etc.) and of extreme humidity. At the workplace, large temperature fluctuations, direct sunlight and vibrations should be avoided. Note: These conditions can distort measurements and If at all possible, avoid touching the lens s
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5. Unpacking the Microscope Transport For shipping or transporting the microscope and its accessory components, the original packaging should be used. As a precaution to prevent damage from vibra- tions, the following components should be dis- assembled and packaged separately: • Unscrew the objectives • Remove the condenser • Remove the coaxial pinion • Remove the lamp housings • Disassemble the burner of 106z lamp housing • Remove all moving or loose parts 15
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6. Assembly 6. Assembling the Microscope The microscope components are logically as- Coaxial Pinion sembled in this order: Note: • Stage • Condenser The coaxial pinion can be mounted on the left- • Fluorescence illuminator* or right-hand side. • Intermediate systems* • Tube • First, place the flat fine focus wheel on the • Eyepieces side to which you intend to mount the coaxial • Objectives pinion; the wheel is held in place magnetically • Lamp housings with light sources* (4.1); ensure that the
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6. Assembly Adjusting the Focus Stop • Loosen the locking screw (5.1) on the left side underside of stage and move the stage all the The focus stop is preadjusted by the factory to way back prevent collision with objectives. The focus stop should be set approx. 0.3 mm higher as the focal • Loosen the screw for coaxial pinion (6.1), plane to enable focussing of the samples of dif- remove the coaxial pinion, and attach the ferent thickness. stage lock with this screw • Loosen the stage lock screw
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6. Assembly 6.2 Condenser • If present, screw the condenser head into the Note: condenser The condenser must be centered before using the microscope. • Using the condenser height adjuster (9.3), turn → Köhler illumination p. 28. the condenser holder (fig. 8) completely downward • Unscrew the clamping screw for the con- denser (9.2) far enough so that the condenser Fig. 8 Condenser holder 1 Guiding notch can be inserted from the front • From the front, insert the condenser into the condenser hold
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6. Assembly 6.3 Tube and Eyepieces • Loosen the clamping screw (11.1) on the stand • Insert the tube in the circular receptacle Note: (dovetail ring) For fluorescence applications, install the fluorescence illuminator* first → p. 21. • Retighten the clamping screw (11.1) If available, the analyzer* (10.1) must be • The eyepieces are inserted into the eyepiece inserted into the stand. This requires that the tubes on the tube guide key engages in the guide pin (10.2). To mount the analyzer, the an
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Leica DM1000
Leica DM1000 LED
Operating Manual · Bedienungsanleitung · Mode d’emploi
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Published June 2010 by/ Herausgegeben Juni 2010 von/ Publiée en june 2010 par : Leica Microsystems CMS GmbH Ernst-Leitz-Strasse 17-37 D-35578 Wetzlar (Germany) Responsible for contents/ Verantwortlich für den Inhalt/ Responsable du contenu rédactionnel : Peter Schmitt, Manuela Jacobsen (Clinical Imaging, Product Management) Holger Grasse (Safety Offi cer according to MPG §30/ Sicherheitsbeauftragter nach MPG §30/ Responsable de la sécurité conformément à la loi relative aux dispositifs médicaux,
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Leica DM1000 Leica DM1000 LED Operating Manual
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Copyrights Copyrights All rights to this documentation are held by Leica Microsystems CMS GmbH. Reproduction of text or illustrations (in whole or in part) by print, photocopy, microfilm or other method (in- cluding electronic systems) is not allowed with- out express written permission from Leica Microsystems CMS GmbH. The instructions contained in the following documentation reflect state-of-the-art technology. We have compiled the texts and illustrations as accurately as possible. Nevertheles
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Contents Contents 8.3 Focusing ...................................................... 36 1. Important Notes about this Manual ...... 6 8.4 Tubes ....................................................... 37 8.5 Eyepieces .................................................... 38 2. Intended Purpose of the Microscope ... 7 8.6 Objectives ................................................... 39 8.7 Light Sources ............................................. 40 3. Safety Notes .............................
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1. Important Notes about this Manual 1. Important Notes about this Manual Caution! This operating manual contains important in- This operating manual is an essential com- structions and information for the operational ponent of the microscope, and must be read safety and maintenance of the microscope and carefully before the microscope is accessories. Therefore, it must be kept and assembled, put into operation or used. taken care of. Text symbols, pictograms and their meanings: (1.2) Numbers i
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2. Intended Purpose of the Microscope 2. Intended Purpose of the Microscope The Leica DM1000/DM1000 LED microscope, to which this user manual belongs, is designed for Caution! biological routine and research applications. This includes the examination of samples taken The manufacturer assumes no liability for from the human body with a view to provide damage caused by, or any risks arising from information on physiological or pathological using the microscope for other purposes states or congeni
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6. Assembly 6.5 Light Source for the Transmitted Light Axis Caution! Note: Light sources pose a potential irradiation risk (glare, UV-radiation, IR-radiation). The Leica DM1000 LED is equipped with Therefore, lamps have to be operated in integrated LED illumination. The service life of closed housings. the LED is about 100.000 hours. If, despite this, it should be necessary to change the LED, this task must be carried out by Technical Service Replacing the Lamp of the Integrated only. Illuminati
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3. Safety Notes 3. Safety Notes 3.1 General Safety Notes 3.2 Electrical Safety This safety class 1 (DM1000) or class 2 General Specifications (DM1000 LED) device is constructed and tested Microscope in accordance with EN 61010-2-101:2002 (DM1000/DM1000 LED), For indoor use only. EN 61010-1:2001 (DM1000/DM1000 LED), Supply voltage: 90-250 V AC, 50-60 Hz IEC 61010-1:2001 (DM1000/DM1000 LED), (DM1000) IEC 60825-1:2007 (DM1000 LED) 12 V DC, 1.5 A EN 60825-1 + A1 + A2:2003 (DM1000 LED), (DM1000 LED)
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3. Safety Notes If the original power supply fails or is Caution! damaged, it must be replaced. Repair is not permitted. Protect the microscope from excessive tem- Original power supplies are available from perature fluctuations. Such fluctuations can your Leica branch office or Leica dealer. lead to the accumulation of condensation, which can damage the electrical and optical components. Ambient temperature: 15-35°C. Caution! The power plug may only be plugged into an outlet equipped with a gro
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4. Overview of the Instrument 4. Overview of the Instrument Specification Leica DM1000/DM1000 LED Contrast Methods • Transmitted light: brightfield, darkfield, phase contrast, polarization • Incident light: fluorescence Transmitted Light Axis Leica DM1000: Integrated halogen illumination Leica DM1000 LED: Integrated LED illumination manual adjustment of • Light intensity • Aperture diaphragm • Field diaphragm (only with Köhler kit) Incident Light Axis Incident light fluorescence illuminator for
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4. Overview of the Instrument Specification Leica DM1000/DM1000 LED Condenser optionally with • CL/PH 0.90/1.25 OIL condenser with color coding • CLP/PH 0.85 condenser for polarization • Achr.apl. A 0.9 (P) condenser with swivelable condenser head • UCL 0.90/1.25 OIL universal condenser UCLP 0.85 for polarization with 5-position light ring disk) • UCL/P pol. universal condenser with interchangeable condenser head and condenser disk with 6 positions Focusing • Focus wheel for coarse and fine focu
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4. Overview of the Instrument 6 5 4 12 3 Fig. 1 Left side of the Leica DM1000 stand 1 Coarse and fine focusing 2 Condenser height adjustment 3 Brightness control 4 Field diaphragm 5 Aperture diaphragm 6 Condenser 12
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4. Overview of the Instrument 1 2 3 4 5 6789 10 Fig. 2 Right side of the Leica DM1000 stand 1 Eyepiece 2 Eyepiece tube 3 Tube 4 Objective turret with objectives 5 Specimen stage with specimen holder 6 Integrated illumination 7 On/Off switch 8 Condenser height adjustment 9 Coarse and fine focusing 10 Coaxial pinion for x/y stage movement 13
