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BioOptix™ 10
Installation and Operation Guide
#69-5203-150
Copyright © 2004 Teledyne Isco, Inc.
All rights reserved.
Revision B, September 20, 2005
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Foreword This instruction manual is designed to help you gain a thorough understanding of the operation of the equipment. Teledyne Isco recommends that you read this manual completely before placing the equipment in service. Although Teledyne Isco designs reliability into all equipment, there is always the possi- bility of a malfunction. This manual may help in diagnosing and repairing the malfunc- tion. If the problem persists, call or e-mail the Teledyne Isco Technical Service Department for a
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™ BioOptix 10 Safety ™ BioOptix 10 Safety General Warnings Before installing, operating, or maintaining this equipment, it is imperative that all hazards and preventive measures are fully understood. While specific hazards may vary according to location and application, take heed in the following general warnings: WARNING Liquids associated with this instrument may be classified as carcinogenic, biohazardous, flammable, or radioactive. Should these liquids be used, it is highly recommended tha
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™ BioOptix 10 Safety AVERTISSEMENT Ce système peut utiliser des dissolvants organiques inflammables. Pour réduire le péril qui peut être causé par l'accumulation des vapeurs explosives, Teledyne Isco recommande que vous installez ce système dans un environnement bien-aéré qui est conçu pour les matières hasardeuses. Cet environnement devrait être construit selon les règlements fédéraux, provinciaux, et locaux. Aussi, il devrait se conformer au plan de votre organisation qui concerne les
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™ BioOptix 10 Safety Hazard Symbols The equipment and this manual use symbols used to warn of hazards. The symbols are explained below. Hazard Symbols Warnings and Cautions The exclamation point within the triangle is a warning sign alerting you of important instructions in the instrument’s technical reference manual. The lightning flash and arrowhead within the triangle is a warning sign alert- ing you of “dangerous voltage” inside the product. Symboles de sécurité Ce symbole signale l’existen
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™ BioOptix 10 Safety vi
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BioOptix™ 10 Table of Contents Section 1 Introduction 1.1 Product Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1 1.2 System Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2 1.3 System Controls and Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3 1.3.1 BioOptix 10 Front Panel Controls . . . . . . . . . . . . . . . . . .
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BioOptix™ 10 Table of Contents 3.8 Post-experiment Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11 3.8.1 Pump Flush . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11 3.8.2 Saving Run Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-12 3.8.3 AutoReports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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BioOptix™ 10 Table of Contents List of Figures 1-1 BioOptix 10 front panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3 1-2 BioOptix 10 left-side connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4 1-3 BioOptix 10 right-side connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5 1-4 BioOptix 10 rear panel controls and connections . . . . . . . . . . . . . . . . . . . .
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BioOptix™ 10 Table of Contents List of Tables 1-1 BioOptix 10 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2 1-2 BioOptix 10 Front Panel Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3 1-3 BioOptix 10 Left-side Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4 1-4 BioOptix 10 Right-side Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
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BioOptix™ 10 Section 1 Introduction 1.1 Product Overview The BioOptix™ 10 system performs automated protein purifi- cation on as many as ten liquid samples simultaneously. The system has ten independent pumps that keep flow rates properly regulated in each channel, and ten independent detectors so that you can monitor the separation in every channel. The purified sample is collected by a fraction collector — up to 300 fractions per sample for unattended operation, or unlimited fractions for atte
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BioOptix™ 10 Section 1 Introduction 1.2 System Specifications Table 1-1 lists the BioOptix 10 specifications. Table 1-1 BioOptix 10 Specifications a Dimensions Height: 69 cm Width: 72 cm Depth: 61 cm a,b Weight 210 kg a,c Power Requirement 100 VAC ±10%, 2.0 Amps 117 VAC ±10%, 2.0 Amps The power switch is the 234 VAC ±10%, 1.0 Amps disconnect device. Line Frequency 50 or 60 Hz a Operating Temperature 5 to 40°C a Humidity When connected to mains power, 95% relative humidity maximum at 5 to
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BioOptix™ 10 Section 1 Introduction 1.3 System Controls and Connectors 1.3.1 BioOptix 10 Front Panel Controls 1 2 3 4 5 Figure 1-1 BioOptix 10 front panel Table 1-2 BioOptix 10 Front Panel Controls Item Name Description 1 Manual Sample Inject Port Injection port for syringe-injected liquid samples. 2 Mode Knob Configures fluid path for purging, manual sample injection, or purification operation. 3 Column Inlet Port Allows connection of tubing to column inlet. 4 Column Clamp Position Adjust
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BioOptix™ 10 Section 1 Introduction 1.3.2 BioOptix 10 Left-side Controls and Connections 1 2 4 3 5 6 7 Figure 1-2 BioOptix 10 left-side connections Table 1-3 BioOptix 10 Left-side Connections Item Name Description 1 Buffer A Inlet Ports Ports to accept Buffer A solution for each channel. 2 Buffer B Inlet Ports Ports to accept Buffer B solution for each channel. 3 Sample In Ports Ports to accept sample for each channel. 4 Pump Purge Port When the Mode Knob is in the Purge Pump position, pumpe
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BioOptix™ 10 Section 1 Introduction 1.3.3 BioOptix 10 Right-side Connections 1 Figure 1-3 BioOptix 10 right-side connections Table 1-4 BioOptix 10 Right-side Connections Item Name Description 1 Purified Sample Outlet Channels 1 through 10 which connect to the Foxy 200 Fraction Collector. 1-5
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BioOptix™ 10 Section 1 Introduction 1.3.4 BioOptix 10 Rear Panel Controls and Connections 1 3 2 4 Figure 1-4 BioOptix 10 rear panel controls and connections Table 1-5 BioOptix 10 Rear Panel Controls and Connections Item Name Description 1 Cooling Fan Ventilates the BioOptix 10 case and cools internal components. 2 External Control Connector Connects to the Foxy 200 Fraction Collector and computer. 3 Fuses Rear Panel Accessible Fuses. Must always be replaced with same type fuses: 100/117 VA
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BioOptix™ 10 Section 2 Installation This section provides instructions for installing the system and preparing it for operation. The BioOptix 10 system consists of a BioOptix 10 unit, a Foxy 200 Fraction Collector, and a Windows PC running BioOptix 10 software. WARNING The system is heavy. Use a two-person lift to prevent injury. 2.1 Unpacking the Carefully unpack the shipment and inspect the contents to verify receipt of all components against the packing list. If there is any BioOptix 10 Sy
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BioOptix™ 10 Section 2 Installation To keep tubing length at a minimum, buffer, sample, and waste collection containers should be placed to the left of the unit. The containers may also be kept behind the unit as long as the con- tainers are accessible. The Foxy 200 should be installed to the right of the BioOptix 10. The fraction collector requires about 1800 square centimeters 2 (275 in ) of bench space. Ensure that the full motion of the fraction collector arm is not obstructed. The Windows P
